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Lab Orders
Regular Lab Supplies
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Orders
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Complete Price Quote
General Methods
Denaturing PAGE Gel
Composition
Denaturing PAGE Gel Power Settings
SDS PAGE
Gel
Preparation
of DH5alpha Competent Cells
Cesium
chloride Plasmid Prep
DNA
Sequencing
Synthetic
DNA Deprotection
Synthetic
RNA Deprotection
Desalting
Oligos with C18 SepPak Column
In
vitro Transcription Reactions
Standard
Transcription Reaction
ASL
Transcription Reaction
tRNA
Transcription Reaction
16S rRNA
Transcription
RNA
Methods
Overexpression
and Purification of Native tRNA Phe
Formylation
and Aminoacylation of tRNA fMet
N-Acetylation
of Aminoacylated tRNA
Determining
the Efficiency of Aminoacylation
Acid-Urea
Gel analysis
Labeling
mRNA with Pyrene
Purifying
Phosphorothioate RNA
Assigning
Rp Vs Sp stereoisomer of Phosphorothioate RNA
Ribosome
Methods
Calculating
the concentration of Ribosome
Purifying
Tight-Couple 70S Ribosomes from E. coli MRE600 (Do NOT USE! see
below)
Purifying
Tight-Couple 70S Ribosome from E. coli MRE600 WITH EXTRA SALT WASH (USE
THIS!!!)
Purifying
30S and 50S Ribosomal Subunits
Subunit
Association
Polysome
Analysis
Purifying
MS2-GST
Purifying
MS2-Tagged 30S subunits
Purifying
U1A-Tagged 50S subunits
Purifying
MS2-Tagged 50S subunits
Determining
the Purity of MS2-Tagged Ribosome
Purifying
TP30 and TP50
Two-Dimensional
Protein Gel Analysis
In vitro
Reconstitution of the 30S subunit
Standard
Chemical Modification of Ribosomes
ENU
Modification of RNA
Fe-BABE
Modification of RNA
Primer Extension Analysis of rRNA
Primers
for 16S and 23S rRNAs
rRNA Sequences: 16S rRNA 16S-MS2 23S rRNA 23S-MS2 pKK3535
Preparation
of S-100 Extract
In vitro
Translation of Renilla Luciferase (see Update 1)
Renilla
Luciferase Update1
Native
Gel Analysis of EF-Tu-GTP-aatRNA Ternary Complex
tRNA
Filter binding
Toeprinting
Assay for Translocation
Attaching
Fluorescent dye to Protein
Protein
Purification
Purification
of His-Tagged T7 RNA Polymerase
Purification
of EF-Tu using IMPACT Method
Purification
of EF-G using IMPACT Method
Purification
of His-Tagged RF1
Protease
Inhibition Guide
Instruments
Scintillation
Counter
Fluoromax
3 Instructions
Rapid
Kinetic Methods
Stopped-Flow
Instructions
Determining
the Dead Time of the Stopped-Flow (Reaction of NATA with NBS)
Rate of Translocation
Rate of EF-Tu ternary complex binding
to Ribosome
Stopped-Flow
Anisotropy Measurement (Tech note 38)
Quench-Flow Instructions
Quench-Flow
Test Reaction (Hydrolysis of DNPA)
Rate of Puromycin Reaction
Rate of fMet-Phe Dipeptide Reaction
Rate of GTP hydrolysis by EF-G
Rate of GTP hydrolysis by EF-Tu Ternary
complex
Analysis
of Kinetic Data
Recommended Reading
Enzyme Kinetics
Johnson (JBC 2008)
Johnson 1992 (The Enzymes Vol XX)
Williamson
(NSMB 2008)
Copeland
(Biochemistry) 2008
Fluorescence
Johnson
1998 (Molecular Probes)
Raines
2008 (ACS Chemical Biology Review) CHART TABLE
Lifetime
of Selected Fluorophores
Company Protocols and Handbooks
Laboratory Bench Guide
RNA
guidelines
Bacterial
growth and storage
Quickchange
In vitro
transcription
Amine
modification
Equilibrium
dialysis
Phosphor
Imager Screen
Fluorescence
Anisotropy
Molecular
Beacon
Protein Purification
IMPACT protein
purification
His
Tagged protein purification
GST
Tagged protein purification
QIAexpressionist
Competent
cells
pET
expression manual
Protein
purification handbook
Recombinant
protein purification handbook
Affinity
purification handbook
Ion exchange purification handbook
Hydrophobic and Reverse phase handbook
Gel filtration handbook
AKTA Purifier Quick Guide
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